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vitronectin  (R&D Systems)


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    Structured Review

    R&D Systems vitronectin
    (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for <t>vitronectin,</t> MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.
    Vitronectin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+clusterin/Mouse+Clusterin+Antibody/bio_rxiv__64898__2026__03__19__712761-240-18-19
    Average 93 stars, based on 45 article reviews
    vitronectin - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation"

    Article Title: Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation

    Journal: bioRxiv

    doi: 10.64898/2026.03.19.712761

    (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.
    Figure Legend Snippet: (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

    Techniques Used: Immunofluorescence, Staining, Fluorescence, Western Blot

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    Membrane:

    Article Title: LRRK2 Kinase Inhibition Attenuates Astrocytic Activation in Response to Amyloid β 1-42 Fibrils
    Article Snippet: .. Subsequently, proteins were transferred on a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), saturated with non-fat dry milk 5% in TBS-Tween 1% (TBST) 1 h at RT and incubated with primary antibodies: anti-β amyloid clone 6E10 (Biolegend, 803004, 1:1000), anti-GAPDH (ThermoFisher Scientific MA5-15738, 1:30,000), anti-IL-1β (R&D System AF-401-NA, 1:2000), anti-LRRK2 phospho serine 935 (Abcam ab133450, 1:300), anti-LRRK2 (Abcam ab133474, MJFF2 1:300), anti-clusterin (R&D System AF2747, 1:2000). .. Next, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck/Sigma Aldrich) for 1 h at RT and then with ECL substrate of HRP.

    Article Title: LRRK2 Kinase Inhibition Attenuates Astrocytic Activation in Response to Amyloid β 1-42 Fibrils.
    Article Snippet: .. Subsequently, proteins were transferred on a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), saturated with non-fat dry milk 5% in TBS-Tween 1% (TBST) 1 h at RT and incubated with primary antibodies: anti-β amyloid clone 6E10 (Biolegend, 803004, 1:1000), anti-GAPDH (ThermoFisher Scientific MA5-15738, 1:30,000), anti-IL-1β (R&D System AF-401-NA, 1:2000), anti-LRRK2 phospho serine 935 (Abcam ab133450, 1:300), anti-LRRK2 (Abcam ab133474, MJFF2 1:300), anti-clusterin (R&D System AF2747, 1:2000). .. Next, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck/Sigma Aldrich) for 1 h at RT and then with ECL substrate of HRP.

    Incubation:

    Article Title: LRRK2 Kinase Inhibition Attenuates Astrocytic Activation in Response to Amyloid β 1-42 Fibrils
    Article Snippet: .. Subsequently, proteins were transferred on a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), saturated with non-fat dry milk 5% in TBS-Tween 1% (TBST) 1 h at RT and incubated with primary antibodies: anti-β amyloid clone 6E10 (Biolegend, 803004, 1:1000), anti-GAPDH (ThermoFisher Scientific MA5-15738, 1:30,000), anti-IL-1β (R&D System AF-401-NA, 1:2000), anti-LRRK2 phospho serine 935 (Abcam ab133450, 1:300), anti-LRRK2 (Abcam ab133474, MJFF2 1:300), anti-clusterin (R&D System AF2747, 1:2000). .. Next, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck/Sigma Aldrich) for 1 h at RT and then with ECL substrate of HRP.

    Article Title: Extracellular clusterin limits the uptake of α‐synuclein fibrils by murine and human astrocytes
    Article Snippet: Then, 15 μg of total proteins were separated by electrophoresis on 7.5% polyacrylamide gels and then transferred on PVDF membrane (Bio‐Rad). .. After saturation with 5% nonfat dry milk, membranes were incubated 1 hr at RT with anti‐GAPDH (ThermoFisher Scientific MA5‐15738, 1:8,000) and anti‐clusterin (R&D Systems AF2747, 1:1,000). .. Subsequently, membranes were incubated 1 hr at RT with HRP conjugated secondary antibodies (Sigma Aldrich) and finally with ECL western blot substrate (GE Healthcare).

    Article Title: Extracellular clusterin limits the uptake of α‐synuclein fibrils by murine and human astrocytes
    Article Snippet: Subsequently, the proteins were transferred on PVDF membrane (Bio‐Rad). .. After saturation with 5% nonfat dry milk, membranes were incubated 1 hr at RT with the following primary antibodies: anti‐GAPDH (ThermoFisher Scientific MA5‐15738, 1:8,000), anti‐α‐synuclein (Abcam, ab138501 MJFR 1:10,000), anti‐clusterin (mouse reactivity; R&D Systems AF2747, 1:1,000), anti‐clusterin (human reactivity; R&D Systems AF2937, 1:1,000), anti‐Glial Fibrillar Acidic Protein (GFAP, GeneTex GTX108711, 1:2,000), anti‐Excitatory Amino Acid Transporter 2 (EAAT2, Novus Biological NBP1‐20136, 1:500). .. Subsequently, membranes were incubated 1 hr at RT with HRP conjugated secondary antibodies (Sigma Aldrich) and finally with ECL western blot substrate (GE Healthcare).

    Article Title: Extracellular clusterin limits the uptake of α‐synuclein fibrils by murine and human astrocytes
    Article Snippet: .. Afterward, primary antibodies anti‐clusterin (R&D Systems, AF2747) and anti‐S100β (Abcam, ab52642) were used at 1:500 and incubated ON at 4°C in 1% NDS, 1% BSA, 0.3% Triton in PBS. ..

    Article Title: LRRK2 Kinase Inhibition Attenuates Astrocytic Activation in Response to Amyloid β 1-42 Fibrils.
    Article Snippet: .. Subsequently, proteins were transferred on a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), saturated with non-fat dry milk 5% in TBS-Tween 1% (TBST) 1 h at RT and incubated with primary antibodies: anti-β amyloid clone 6E10 (Biolegend, 803004, 1:1000), anti-GAPDH (ThermoFisher Scientific MA5-15738, 1:30,000), anti-IL-1β (R&D System AF-401-NA, 1:2000), anti-LRRK2 phospho serine 935 (Abcam ab133450, 1:300), anti-LRRK2 (Abcam ab133474, MJFF2 1:300), anti-clusterin (R&D System AF2747, 1:2000). .. Next, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck/Sigma Aldrich) for 1 h at RT and then with ECL substrate of HRP.

    Article Title: Extracellular clusterin limits the uptake of α‐synuclein fibrils by murine and human astrocytes
    Article Snippet: .. The media collected after α‐synuclein pffs treatment were incubated with 15 μl of Dynabeads Protein G (ThermoFisher Scientific, 10007D) and 1 μg of anti‐clusterin (R&D Systems AF2747) 2 hr at 4°C on a rotating wheel. ..



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    (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and <t>clusterin.</t> Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.
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    Image Search Results


    (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

    Journal: bioRxiv

    Article Title: Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation

    doi: 10.64898/2026.03.19.712761

    Figure Lengend Snippet: (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

    Article Snippet: Antibodies against LC3B (Sigma, L8918-200UL), actin (Proteintech, 66009-1-Ig), PLIN2 (Proteintech, 15294-1-AP), fibronectin (Invitrogen, PA5-29578), clusterin (R&D systems, AF2747), vitronectin (R&D Systems, MAB38751), and p62 (Abnova, H00008878-M01; CST, 5114), Lamp2a (Abcam, ab125068), Lamp2b (Abcam, ab13524), Iba1 (Fujifilm Wako, 019-19741), Laminin (Sigma, L9393) and ApoE (Abcam, ab183596) were used in this study.

    Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot

    (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

    Journal: bioRxiv

    Article Title: Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation

    doi: 10.64898/2026.03.19.712761

    Figure Lengend Snippet: (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

    Article Snippet: Antibodies against LC3B (Sigma, L8918-200UL), actin (Proteintech, 66009-1-Ig), PLIN2 (Proteintech, 15294-1-AP), fibronectin (Invitrogen, PA5-29578), clusterin (R&D systems, AF2747), vitronectin (R&D Systems, MAB38751), and p62 (Abnova, H00008878-M01; CST, 5114), Lamp2a (Abcam, ab125068), Lamp2b (Abcam, ab13524), Iba1 (Fujifilm Wako, 019-19741), Laminin (Sigma, L9393) and ApoE (Abcam, ab183596) were used in this study.

    Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot